Genetic optimization for high-yield carotenoids #worldresearchawards
Description Metabolic engineering of Escherichia coli has emerged as a powerful strategy for sustainable β-carotene production. By introducing and optimizing heterologous carotenoid biosynthetic pathways, researchers enhance precursor availability through the mevalonate (MVA) or methylerythritol phosphate (MEP) pathways. Key strategies include overexpression of rate-limiting enzymes, elimination of competing pathways, codon optimization, promoter engineering, and balancing metabolic flux to increase isoprenoid precursors such as IPP and DMAPP. Advanced tools like CRISPR-based genome editing, synthetic biology circuits, and systems-level metabolic modeling further improve yield and stability. Process optimization, including fermentation control and cofactor balancing, significantly boosts productivity. Engineered E. coli cell factories provide a cost-effective, scalable, and eco-friendly alternative to chemical synthesis and plant extraction, supporting applications in food, pharmaceut...